Journal: ACS Chemical Neuroscience

Article Title: Oleocanthal Enhances Amyloid-β Clearance from the Brains of TgSwDI Mice and in Vitro across a Human Blood-Brain Barrier Model

doi: 10.1021/acschemneuro.5b00190

Figure Lengend Snippet: Oleocanthal (Oleo) treatment reduces astrocytes activation in the hippocampus of TgSwDI and brain IL-1β. (A) Representative hippocampus sections double-stained with GFAP antibody and anti-Aβ 6E10 to detect activated astrocytes and Aβ load, respectively. Arrows in normal saline group (NS) indicate activated astrocytes with long and thick branches (seen at higher magnification in the closed inserts). (B) Quantitative analysis of GFAP optical density showed a significant reduction in astrocytes activation associated with reduced Aβ levels. Data is presented as mean ± SEM of 4–6 mice in each group. Scale bar, 50 μm. (C) Oleo treatment reduced brain levels of IL-1β ( n = 4/group). (* P < 0.05 and ** P < 0.01). TgSwDI mice were treated with oleocanthal (5 mg/kg/day) or NS for 4 weeks beginning at age 4 months.

Article Snippet: For detection of IL1-β levels in brain homogenate, anti-mouse IL-1 beta Quantikine ELISA kit (R&D Systems, MN) was used according to the manufacturer’s instructions.

Techniques: Activation Assay, Staining, Saline